A Strain of Pediococcus cerevisiae which requires Methicillin for Growth
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چکیده
منابع مشابه
Studies on a mutant strain of Escherichia coli which requires both methionine and lysine for growth.
A mutant of Esckch ia coZi waa isolated which showed a requirement for methionine + lysine when a fairly large inoculum of washed organisms was used. Cystathionine or homocysteine replaced methionine, and diaminopimelic acid replaced lysine; no other amino acid or growth factor was active. The amounts of the amino acids required to promote growth were only about one-tenth of the quantity of met...
متن کاملPlasmid DNA in Strains of Pediococcus cerevisiae and Pediococcus pentosaceus.
Five parental strains of Pediococcus were examined for plasmid content. Each strain contained three to six resident plasmids, ranging in size from 4.5 to 39.5 megadaltons. A bacteriocin-like substance produced by Pediococcus cerevisiae FBB63 was tentatively linked to a 10.5-megadalton plasmid after being cured with novobiocin.
متن کاملUptake and metabolism of folate by a folate-utilizing mutant of Pediococcus cerevisiae (8081-S) and the parent strain (8081).
Pediococcus cerevisiae 8081, which uses folinic acid for growth, and its nutritional mutant 8081/S, which utilizes folate, were compared with respect to their ability to take up and metabolize folate. In contrast to the parent strain 8081, which takes up very little folate, the uptake of folate by the mutant is a concentrative, saturable, and energydependent process. At low extracellular concen...
متن کاملEffect of folinate on thymidine uptake by Pediococcus cerevisiae.
Uptake of (3)H-thymidine by resting cells of Pediococcus cerevisiae was found to be energy- and temperature-dependent. The pH optimum was between 6.5 and 8.0, and after 2 min of incubation most of the radioactivity was found in the deoxyribonucleic acid (DNA) fraction. Iodoacetate at a concentration of 10(-2)m caused a 50% inhibition of uptake. Preincubation of resting cells for 10 min with fol...
متن کاملTransport of folinate and related compounds in Pediococcus cerevisiae.
The properties of folinate and 5-methyltetrahydrofolate (5-CH(3)-H(4)PteGlu) transport mechanism of Pediococcus cerevisiae were studied. The uptake was dependent on temperature, pH (optimum for both compounds at pH 6.0), and glucose. Iodoacetate, potassium fluoride, and sodium azide inhibited the uptake. 5-CH(3)-H(4)-PteGlu was apparently not metabolized but folinate was metabolized. Metabolism...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1968
ISSN: 0022-1287
DOI: 10.1099/00221287-50-1-85